Hito CryoMyelinStain™ Nissl Kit (Gold phosphate complex Myelin Staining Kit with Nissl Counter Stain) is made in a ready-to-use format and offers high quality, rapid staining of myelin/myelinated axons and nuclear counterstaining on frozen sections (mounted or floating). This kit has many advantages compared to the traditional Luxol fast blue staining method which is time-consuming, requires usage of 40-56°C incubator, and usually has low yields of stained myelin fibers and unreliable results. In addition, the long time high temperature incubation process of the frozen sections may cause sections felling off from the slides. Hito CryoMyelinStain™ Nissl Kit offers a simple solution to these problems. The procedures are simplified and the processing time is greatly reduced. Users can use mounted sections or floating sections at room temperature. This kit delivers stable and improved staining quality. It has been proven to be extremely reliable and sensitive for demonstrating the morphological details of myelin fibers.

Myelin is essential for the proper functioning of the nervous system. Demyelination impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Hito CryoMyelinStain™ Nissl Kit allows sensitive localization and visualization of the myelin fibers, thus offers a fast and reliable way to determine the extent of  demyelination.

Hito CryoMyelinStain™ Nissl Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.

Kit Contents (for 60 slides or 200 sections)
Solution-1	50 ml
Solution-2	3 ml
Solution-3	12 ml
Solution-4	250 ml
Solution-5	25 ml
Solution-6	30 ml
Staining Jar	3
Hito Aqua Barrier PAP Pen (HTHS0110)	1
Fine Tip Natural Hair Brush	1
Glass Specimen Transfer Tool	1
User Manual and MSDS	1
Before using Hito CryoMyelinStain Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat and light microscopeDry ice, isopentane, O.C.T. compound, ethanol, xylene, 4% PFA (recommend Hito Buffered 4% Paraformaldehyde Solution Cat# HTSHS0102), double distilled or deionized waterGelatin coated slides and coverslipsStaining jars for slides washResinous mounting medium Hito CryoMyelinStain™ Nissl Kit Manual and MSDS