Hito Verhoeff OptimStain™ Kit is designed based on the Van Gieson and Verhoeff staining method with improved and simplified procedures. This kit can be used for simultaneously demonstrating the morphological details of collagen and elastic fibers.One example application of this kit is to determine the extent of atherosclerosis. The atherosclerotic plaque contains lipids, inflammatory cells, smooth muscle cells, connective tissue (eg, collagen, glycosaminoglycans, elastic fibers), thrombi, and calcium deposits. Fibrillar collagen is a critical component of atherosclerotic lesions. Uncontrolled collagen accumulation leads to arterial stenosis, while excessive collagen breakdown combined with inadequate synthesis weakens plaques thereby making them prone to rupture. With its special design, Hito Verhoeff OptimStain™ Kit will allow localization and visualization of the collagen and damage of elastic structure, therefore, enables a good understanding of the extent of atherosclerosis. This kit can also be used to demonstrate changes in elastic tissues in case of emphysema and other vascular diseases.Hito Verhoeff OptimStain™ Kit is made in a ready-to-use format and provides high quality, reliable and sensitive staining of collagen and elastic fibers.Hito Verhoeff OptimStain™ Kit has been tested extensively on the hearts and arteries from several species of animals and it is a simple solution for your research.

Kit Contents (for >150 slides)
Solution-1A	125 ml
Solution-1B	55 ml
Solution-1C	55 ml
Solution-2	225 ml
Solution-3	225 ml
Solution-4	225 ml
Solution-5	225 ml
Staining jars	5
User Manual and MSDS	1
Before using Hito Verhoeff OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or Microtome, Light microscopeParaffin embedding equipment (or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSH0102, for paraffin sections preparation)Dry ice, isopentane, O.C.T. compound (for frozen sections), 4% PFA (Cat# HTSH0101), ethanol, xylene, double distilled or deionized waterSlides, coverslipsStaining jars for slides washResinous mounting medium  Hito Verhoeff OptimStain™ Kit Manual and MSDS